To estimate the hemoglobin concentration in a blood sample using the Sahli’s acid hematin method, providing a quantitative measure for assessing physiological and pathological conditions like anemia.

Hemoglobin is converted into Acid Hematin through the action of dilute Hydrochloric Acid (0.1N HCl). The acid lyses the erythrocytes and reacts with the hemoglobin to produce a dark brown solution. This solution is subsequently diluted with distilled water until its color intensity matches the standard brown glass plates of the Sahli's comparator box.

Sahli’s Hemoglobinometer kit (Comparator box with brown glass standards), Sahli’s graduated hemoglobin tube, Sahli’s pipette (20 microliters), 0.1N HCl, distilled water, glass stirrer, sterile lancet, and alcohol swabs.

1. Initial Acidification: Add 0.1N HCl into the Sahli’s graduated tube up to the 2 g/dL mark.
2. Blood Transfer: Obtain 20 microliters of blood using the Hb pipette and transfer it into the tube. Carefully rinse the pipette with the acid mixture.
3. Incubation: Mix with the stirrer and allow the tube to stand for 10 minutes for complete conversion of Hb to acid hematin.
4. Titration: Add distilled water drop by drop, stirring continuously, until the color perfectly matches the standard glass plates.
5. Reading: Note the reading of the lower meniscus on the gram-percent (g%) scale.